anti irf3 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology anti irf3
Anti Irf3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3/IRF-3+Antibody/pm42829916-401-31-34
Average 96 stars, based on 750 article reviews

anti irf3 - by Bioz Stars, 2026-11

96/100 stars

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Bioprocessing:

Article Title: Megakaryocytic Differentiation Regulates the Permissiveness and Antiviral Response of the Megakaryocytic Erythroid Progenitor to Dengue Virus
Article Snippet: .. For the detection of OAS2 and IRF3 proteins, primary monoclonal antibodies against OAS2 and IRF3 (both from Santa Cruz Biotechnology, Dallas, TX, USA) were used at a dilution of 1:200 under the same incubation conditions. ..

Incubation:

other:

Article Title: Elongator is required for pattern recognition receptor and type I interferon signaling in macrophages
Article Snippet: IRF3 , Rabbit , Santa cruz , sc-9082 , 1:1000.

Article Title: Chloroquine enhances MAVS-mediated cytokine induction and alters mitochondrial morphology.
Article Snippet: Equivalent amounts of proteins were separated on SDS-PAGE and transferred to a nitrocellulose membrane (XR-IGE-10600003, Amersham).

Article Title: Chloroquine Enhances Mitochondrial Antiviral Signaling-Mediated Cytokine Induction and Alters Mitochondrial Morphology
Article Snippet: Equivalent amounts of proteins were separated on SDS-PAGE and transferred to a nitrocellulose membrane (XR-IGE-10600003, Amersham).

Article Title: HTATSF1 regulates innate antiviral immune response by orchestrating TRAF3-IRF3 and TRAF6-NF-κB pathways
Article Snippet: 2′3′-cGAMP (tlrl-nacga23-02, Invitrogen); lipofectamine 2000 (52887, Invitrogen); polybrene (TR-1003-G, Millipore); puromycin (Thermo); RNAiso plus (9109, Takara Bio); SYBR Green Mix (172–5274, Bio–Rad); geneticin (G-418) (11811-031, Gibco); ELISA kits for murine IFN-β (42400, PBL), CXCL10 (EMC121, NeoBioscience), IL-6 (431304, BioLegend); recombinant hIFN-β (300-02BC, PEPRO TECH); poly(I:C) (tlrl-pic-5, Invitrogen); herring testis (HT)-DNA (D6898, Sigma); human IL-1β (211-11B-100, PEPRO TECH) were purchased from the indicated companies.

Immunopeptidomics:

Article Title: NLRC5 Regulates Enterovirus 71 Infection Through an IFN-β-Dependent Pathway
Article Snippet: .. Subsequently, the membranes were blocked with 5% skim milk for 2 h at room temperature before being probed with specific primary antibodies, such as those targeting NLRC5 (1:1000, sc-515888, Santa Cruz, CA, USA), RIG-I (1:1000, sc-376845, Santa Cruz, CA, USA), IRF3 (1:1000, sc-33641, Santa Cruz, CA, USA), phospho-STAT1 (1:1000, sc-8394, Santa Cruz, CA, USA), MHC-I (1:1000, sc32235, Santa Cruz, CA, USA), DYKDDDDK (1:1000, 66008-4-Ig, Proteintech, Wuhan, China), β-actin (1:5000, 66009-1-Ig, Proteintech, Chicago, IL, USA), and VP1 (1:1000, NZK-A14002, hbnzk, Hubei, China) overnight at 4 °C. .. The membrane was washed with TBST (50 mM Tris Base, 0.15 M NaCl, 2.7 mM KCl, 0.1% Tween-20 (ST2789, Shanghai, China)) 3 times, 10 min/time, and incubated with secondary antibody (1:10,000, Proteintech, Wuhan, China) for 1 h at room temperature.

Article Title: NLRC5 Regulates Enterovirus 71 Infection Through an IFN-β-Dependent Pathway.
Article Snippet: .. Subsequently, the membranes were blocked with 5% skim milk for 2 h at room temperature before being probed with specific primary antibodies, such as those targeting NLRC5 (1:1000, sc-515888, Santa Cruz, CA, USA), RIG-I (1:1000, sc-376845, Santa Cruz, CA, USA), IRF3 (1:1000, sc-33641, Santa Cruz, CA, USA), phospho-STAT1 (1:1000, sc-8394, Santa Cruz, CA, USA), MHC-I (1:1000, sc32235, Santa Cruz, CA, USA), DYKDDDDK (1:1000, 66008-4-Ig, Proteintech, Wuhan, China), β-actin (1:5000, 66009-1-Ig, Proteintech, Chicago, IL, USA), and VP1 (1:1000, NZK-A14002, hbnzk, Hubei, China) overnight at 4 ◦C. .. The membrane was washed with TBST (50 mM Tris Base, 0.15 M NaCl, 2.7 mM KCl, 0.1% Tween-20 (ST2789, Shanghai, China)) 3 times, 10 min/time, and incubated with secondary antibody (1:10,000, Proteintech, Wuhan, China) for 1 h at room temperature.

Ubiquitin Proteomics:

Article Title: HTATSF1 regulates innate antiviral immune response by orchestrating TRAF3-IRF3 and TRAF6-NF-κB pathways.
Article Snippet: 2′3′-cGAMP (tlrl-nacga23-02, Invitrogen); lipofectamine 2000 (52887, Invitrogen); polybrene (TR-1003-G, Millipore); puromycin (Thermo); RNAiso plus (9109, Takara Bio); SYBR Green Mix (172–5274, Bio–Rad); geneticin (G-418) (11811-031, Gibco); ELISA kits for murine IFN-β (42400, PBL), CXCL10 (EMC121, NeoBioscience), IL-6 (431304, BioLegend); recombinant hIFN-β (300-02BC, PEPRO TECH); poly(I:C) (tlrl-pic-5, Invitrogen); herring testis (HT)-DNA (D6898, Sigma); human IL-1β (211-11B-100, PEPRO TECH) were purchased from the indicated companies. .. Antibodies against HA (TA180128) (OriGene); Flag (F3165) and β-actin (A2228) (Sigma); p-TBK1 S172 (ab109272), TBK1 (ab40676), pIRF3 S386 (ab76493), TAK1 (ab109526), TRAF6 (ab33915), p65 (ab7970), ubiquitin (ab7254), K48-linkage specific polyubiquitin (ab140601) and K63-linkage specific polyubiquitin (ab179434) (Abcam); IRF3 (sc-33641) and STAT1 (SC-417) (Santa Cruz Biotechnology); Myc (5605), p-IRF3 S396 (4947), p-STAT1 Y701 (9167), TRAF3 (4729), p-TAK1 T184/187 (4508), p-IκBα S32/36 (9246), IκBα (9242), pIKKα/β S176/177 (2078), IKKβ (2370), and p-p65 S536 (3033) (Cell Signaling Technology), HTATSF1 (20805-1-AP) and HECTD3 (11487-1- AP) (Proteintech) were purchased from the indicated companies. .. HEK293 cells and THP-1 cells were obtained from ATCC.

Review